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Culturing primary cells has offered insights into cellular pathways and disease pathogenesis. The similarities that are shared with tissue have promoted the use of these cells in research. The data from primary cell culture research is reproducible in the in vivo models. Recent studies have been divulging the difference between genome, transcriptome, and signaling pathways of cells from different tissues. It has further promoted the isolation and culture of primary cells from different tissues and species, adding precision to research.

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